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Sample: Blood
Depending on the “gold standard” and other methodological factors, studies show PCR sensitivities ranging from 77% to more than 95% and PCR specificities of >95% for smear-positive specimens (4, 9, 10, 12). However, sensitivities for smear-negative TB patients have been reported to be below 90% (9).
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TB RT PCR on tissue block
How accurate is PCR test for TB?
tuberculosis. Most reports have evaluated PCR using known AFB-positive samples17,18,19. In smear-positive specimens, the sensitivity and specificity of PCR are in the range 90%-100%, with a positive predictive value of >95%, whereas in smear-negative specimens, the sensitivity of PCR is reduced to <50%17
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